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axioobserver inverted widefield microscope (Carl Zeiss)

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Carl Zeiss axioobserver inverted widefield microscope
Axioobserver Inverted Widefield Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 4867 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/axio+observer+widefield+microscope/Inverted+microscope+Axio+Observer+3/pm41896195-224-10-9
Average 99 stars, based on 4867 article reviews

axioobserver inverted widefield microscope - by Bioz Stars, 2026-09

99/100 stars

Images

3D Imaging:

Article Title: Spatial and Temporal Single-Cell Profiling of RNA Compartmentalization in Neurons with Nanotweezers.
Article Snippet: For MAP1A smFISH, cells were stained with Wheat Germ Agglutin (WGA) conjugated to Alexa Fluor 647 (Invitrogen) to stain the neuronal structure before mounting. .. Three-dimensional (3D) imaging was performed on a Leica Stellaris 8 confocal microscope or a Zeiss Axio Observer widefield microscope. ..

Article Title: Spatial and Temporal Single-Cell Profiling of RNA Compartmentalization in Neurons with Nanotweezers
Article Snippet: After all targets were amplified and conjugated with a fluorophore, the samples were counterstained with DAPI (Bio-Techne) and mounted onto microscope slides (VWR) using ProLong Gold Antifade Mountant with DAPI (Invitrogen). .. Three-dimensional (3D) imaging was performed on a Leica Stellaris 8 confocal microscope or a Zeiss Axio Observer widefield microscope. ..

Microscopy:

Article Title: Virus-associated inflammation imprints an inflammatory profile on monocyte-derived macrophages in the human liver
Article Snippet: Finally, the slides were DAPI stained and mounted with 145 ProLong Gold antifade mountant (Thermo Fisher) for imaging. .. 146 147 Image acquisition, pre-processing and analysis 148 Images were acquired using a Zeiss Axio Observer Widefield microscope (10x and 40x 149 objectives). ..

Article Title: Spatial and Temporal Single-Cell Profiling of RNA Compartmentalization in Neurons with Nanotweezers
Article Snippet: A secondary antibody diluted 1:1000 in blocking buffer was added to the samples for 2–3 h. Cells were washed in PBS and then mounted using ProLong Gold Antifade Mountant with DAPI (Invitrogen). .. Imaging was performed on a Zeiss Axio Observer widefield microscope. ..

Article Title: Mechanisms of nuclear segregation in a multinucleate multibudding yeast
Article Snippet: .. To measure bud-neck widths, cells expressing Cdc3-tdTomato were imaged on a Zeiss Axio Observer widefield microscope using a 63x oil objective, and a Hamamatsu Flash 4 camera. ..

Article Title: Prolonged loss of nuclear HMGB1 in neurons following modeled TBI and implications for long-term genetic health.
Article Snippet: .. A Zeiss Axio Observer widefield microscope with deconvolution was used to acquire images of the region of interest in tissue samples. ..

Article Title: SNX10 functions as a modulator of piecemeal mitophagy and mitochondrial bioenergetics.
Article Snippet: Trachsel-Moncho et al. Journal of Cell Biology S1 SNX10 modulates mitochondrial homeostasis https://doi.org/10.1083/jcb.202404009 D ow nloaded from http://rupress.org/jcb/article-pdf/224/5/e202404009/1940338/jcb_202404009.pdf by N epal user on 13 M arch 2025 Figure S1. .. SNX10 localizes to endocytic structures. (A) Fluorescence imaging of U2OS cell lines stably expressing doxycycline-inducible SNX10-EGFP WT or the indicated ARO-linked mutants was acquired at 20× magnification using a Zeiss Axio Observer widefield microscope (Zen Blue 2.3; Zeiss). ..

Imaging:

other:

Article Title: Spatial and Temporal Single-Cell Profiling of RNA Compartmentalization in Neurons with Nanotweezers.
Article Snippet: Cells were then permeabilized with 0.1% Triton-X-100 (Thermo Scientific) in PBS for 15 min.

Expressing:

Fluorescence:

Stable Transfection:

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Image Search Results

$A 2D widefield immunofluorescence assay (IFA) images of C-WT parasites showing HA-tagged Pf SLIRP in schizonts at the periphery of rhoptry bulbs. EXP2 marks the parasitophorous vacuole membrane, RhopH3 marks rhoptry bulbs, and DNA is stained with DAPI. Super-Resolution Radial Fluctuations (SRRF) processing was applied to enhance spatial resolution. The white box in the merged schizont image indicate zoomed area to the right. B Confocal maximum intensity projections of expanded C-WT schizonts illustrating wild-type Pf SLIRP–HA localisation at the rhoptry membrane. RAP1 marks rhoptry bulbs, GAP45 labels the merozoite periphery, and SYTOX™ blue stains DNA. The white boxes in the merged image indicate zoomed areas at the bottom. B = rhoptry bulb, N = rhoptry neck. C Confocal maximum intensity projections of rhoptries from expanded C-C36W schizonts (Figure S11) illustrating C36W Pf SLIRP-HA localisation at the rhoptry membrane. D Western blot of parasite proteins from ring (R), early trophozoite (ET), late trophozoite (LT), and schizont stages, probed with anti-HA to detect Pf SLIRP. HSP70-1 serves as a loading control. Densitometry values were normalised to HSP70-1, a constitutively-expressed housekeeping protein. Pf SLIRP expression peaks at the schizont stage for C-WT, and the ring stage for C-C36W. Western blot is representative of three biological replicates (refer to Figure S12 for all replicates). Error bars represent the standard deviation of the mean of three biological replicates, each with two technical replicates. Statistical analyses were performed with GraphPad Prism 10, with Welch’s t -test between the late trophozoite and schizont stages within both parasite lines, and between the same stages across both parasite lines. ** p < 0.01, **** p < 0.0001. No bar indicates not significant. E Sequential lysis of C-WT and C-C36W schizont saponin pellets by freeze–thaw and sodium carbonate extraction. Western blot shows Pf SLIRP is found in the carbonate soluble (Na2CO3 Sup.) and insoluble (Na2CO3 Pel.) fractions but not in the PBS soluble fraction (PBS Sup.). Control proteins included soluble protein Pf HSP70-1, peripheral membrane protein Pf HSP101, and integral membrane protein Pf EXP2. Mouse anti-HA was used for Pf SLIRP detection; rabbit antibodies were used to detect the control proteins. * denotes cross-reactive bands produced by rabbit HSP70-1 antibody (refer to Figure S14 for all cross-reactive bands produced by the antibody).$

Journal: bioRxiv

Article Title: MMV687794 blocks Plasmodium falciparum invasion of red blood cells by targeting a Surface-associated Lipid-Interacting Rhoptry Protein, Pf SLIRP

doi: 10.64898/2026.02.24.707847

Figure Lengend Snippet: A 2D widefield immunofluorescence assay (IFA) images of C-WT parasites showing HA-tagged Pf SLIRP in schizonts at the periphery of rhoptry bulbs. EXP2 marks the parasitophorous vacuole membrane, RhopH3 marks rhoptry bulbs, and DNA is stained with DAPI. Super-Resolution Radial Fluctuations (SRRF) processing was applied to enhance spatial resolution. The white box in the merged schizont image indicate zoomed area to the right. B Confocal maximum intensity projections of expanded C-WT schizonts illustrating wild-type Pf SLIRP–HA localisation at the rhoptry membrane. RAP1 marks rhoptry bulbs, GAP45 labels the merozoite periphery, and SYTOX™ blue stains DNA. The white boxes in the merged image indicate zoomed areas at the bottom. B = rhoptry bulb, N = rhoptry neck. C Confocal maximum intensity projections of rhoptries from expanded C-C36W schizonts (Figure S11) illustrating C36W Pf SLIRP-HA localisation at the rhoptry membrane. D Western blot of parasite proteins from ring (R), early trophozoite (ET), late trophozoite (LT), and schizont stages, probed with anti-HA to detect Pf SLIRP. HSP70-1 serves as a loading control. Densitometry values were normalised to HSP70-1, a constitutively-expressed housekeeping protein. Pf SLIRP expression peaks at the schizont stage for C-WT, and the ring stage for C-C36W. Western blot is representative of three biological replicates (refer to Figure S12 for all replicates). Error bars represent the standard deviation of the mean of three biological replicates, each with two technical replicates. Statistical analyses were performed with GraphPad Prism 10, with Welch’s t -test between the late trophozoite and schizont stages within both parasite lines, and between the same stages across both parasite lines. ** p < 0.01, **** p < 0.0001. No bar indicates not significant. E Sequential lysis of C-WT and C-C36W schizont saponin pellets by freeze–thaw and sodium carbonate extraction. Western blot shows Pf SLIRP is found in the carbonate soluble (Na2CO3 Sup.) and insoluble (Na2CO3 Pel.) fractions but not in the PBS soluble fraction (PBS Sup.). Control proteins included soluble protein Pf HSP70-1, peripheral membrane protein Pf HSP101, and integral membrane protein Pf EXP2. Mouse anti-HA was used for Pf SLIRP detection; rabbit antibodies were used to detect the control proteins. * denotes cross-reactive bands produced by rabbit HSP70-1 antibody (refer to Figure S14 for all cross-reactive bands produced by the antibody).

Article Snippet: Images were captured using a Zeiss Axio Observer Z1 inverted widefield microscope with a Plan-Apochromat 100×/1.40 Oil DIC objective and the super-resolution radial fluctuations (SRRF) algorithm was used to capture super-resolution images.

Techniques: Immunofluorescence, Membrane, Staining, Western Blot, Control, Expressing, Standard Deviation, Lysis, Extraction, Produced